The Princess Bride:
I like many other LOVE this movie. "Have fun storming the castle" Is what's used most.
Until recently when I had a pycho customer come in and call me a liar. Now a co-worker and I often shot it at each other just to fun, "LIAR!"
Top Gun:
"That's a negative, Ghost Rider, the pattern is full" Can't believe no one (at least not that I saw) mentioned this one. I use it ALL the time. Usually when someone asks for something there's NO way they'd ever get.
Pirates:
"Ello poppet." Again I'm really surprised no one mentioned this one either. It's used as a greeting with one of my favorite co-workers.
Animated Alice in Wonderland:
"Lose something?" For those who aren't die hard fans like myself the Cheshire cat says this line while Alice is looking for him trying to figure out which way to go next in Wonderland. I usually say it when I find people being nosy.
"Who's been painting my roses RED?" I often use this on at work when co-workers mess with or move something. Which is often.
Live Action Alice in Wonderland:
"I shall futterwhackin - vigorously." It's a shortened part of the actual quote. But oh so funny.
"But I do not owe you a kindness" White Queen says this line to the Nave when he asks her to kill him instead of having a life of exile with the Red Queen. Very few get the reference, but it still amuses me all the same.
"All the best people are." Another shortened quote - I always say it when people rhetorically ask if they are going crazy.
I'm sure there are many many more... those are all I could think of off the top of my head.